Some Applications of the Colorimetric Phosphate Method

نویسنده

  • A. P. BRIGGS
چکیده

Two years ago, a modification (1) of the Bell-Doisy phosphate method was published and at that time technique ‘was given for its application to inorganic phosphorus in blood and urine. Since then its use has been extended to a variety of analytical procedures, some of which are given below. In principle the method consists of the formation of phosphomolybdic acid and its subsequent reduction by hydroquinone and sulfur dioxide with the production of a stable blue color, proportional to the inorganic phosphorus present. The excess molybdic acid is not reduced. Reagents.-The following reagents are used in all of the procedures to be given. Molybdate Solution.-This contains 5 per cent ammonium molybdate in 5 N HzS04, and is prepared as follows: Dissolve 25 gm. of ammonium molybdate in 300 cc. of water and to this add 75 cc. of concentrated H&SO4 diluted with 125 cc. of water. Hydroquinone Solution.-This is a 1 per cent solution to which a drop of concentrated HzS04 is added to retard oxidation. Sulfite Solution.-A 20 per cent solution of sodium sulfite is used. The strength of sulfite solutions deteriorates by oxidation, but the amount used in color development is sufficient provided an easily detectable odor of SOZ is evolved. Stock KH2PO4 Solution.-This is prepared from pure KHzPOd which has been pulverized and dried for several days over concentrated HzS04. 4.394 gm. are dissolved in a liter of distilled water and then 5 cc. of CHCb added as preservative. 1 cc. = 1 mg. of phosphorus.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Performing a Calcium-Phosphate Layer on Porous NiTi Alloy for Using in Orthopedic Applications

Porous NiTi alloys has a series of unique properties such as shape memory effect, superelastic behavior and energy absorbability that make them usable in a wide range of medical and industrial appliances. But the more probability of Ni release from a porous NiTi compare to the nonporous one has restricted it’s uses in implants. In the present research for resolving the problem, performing a cal...

متن کامل

A New Colorimetric Azo-azomethine Probe for Fluoride Ion Detection Based on the Proton Transfer Signaling Mode: Real-life Applications

Four novel receptors were designed and synthesized for colorimetric detection of F− ions. The introduction of four electron withdrawing groups into the backbone of the receptors makes the two phenolic groups efficient hydrogen bonding sites. The binding properties of receptors with anions were examined for the first time by UV–Vis, 1H NMR and fluorescence spectroscopies. The addition of F− resu...

متن کامل

Effect of organic and inorganic additives on some chemical properties of vermicompost, earthworm’s biomass and reproduction

ABSTRACT-The enrichment of bedding for vermicomposting systems with organic and inorganic additives is a technique which can promote the quality of produced vermicompost. Phosphate rock and fishmeal are two available and low-cost substances which can be provided easily and inoculation of vermicompost with phosphate solubilizing microorganisms (PSMs) can enhance the availability...

متن کامل

The Potentials and Applications of Cellulose Acetate in biosensor technology

The interest in cellulose and its derivatives has been exponentially increasing due to its excellent thermal stability, biocompatibility, chemical persistence and biodegradability. Among various cellulose derivatives, cellulose acetate (CA) has been applied in many applications including sensor systems, drug delivery systems, separation membrane, and tissue engineering. Recently, the electrospu...

متن کامل

Performing a Calcium-Phosphate Layer on Porous NiTi Alloy for Using in Orthopedic Applications

Porous NiTi alloys has a series of unique properties such as shape memory effect, superelastic behavior and energy absorbability that make them usable in a wide range of medical and industrial appliances. But the more probability of Ni release from a porous NiTi compare to the nonporous one has restricted it’s uses in implants. In the present research for resolving the problem, performing a cal...

متن کامل

An improved method for the colorimetric determination of phosphate.

ALL the known colorimetric methods for the determination of phosphate are liable to errors arising from (a) slight changes in acidity and concentration of reagents, and (b) the presence of interfering substances (e.g. fluorides, nitrites etc.). The following method is almost entirely independent of these disturbing factors, and possesses the added advantage of being more sensitive than those pr...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2003